Anyone who has seen my home knows that I have a bit of a… hmm… obsession with orchids. To control my orchid intake, I meter my purchases to buying more plants only after I have either mastered or killed my latest orchid purchase. This has resulted in nearly 20 pots of these gorgeous plants with orchid “spa treatment days” (i.e., deep watering days) lasting hours at a time. So you can probably imagine the enthusiasm with which I embraced a recent article from the journal Science, in which Harvard researchers Wim L Noorduin and colleagues proclaim that they have discovered how to create predictable complex nano- and microstructures via biomineralization techniques, and then demonstrate this by creating micro-flowers!
Self-assembly of complex forms occur regularly in nature as a result of dynamic interactions with the surrounding environment. Many of these structures have a stunning beauty to them, with snowflakes being one of the most well-known examples of this phenomenon. The claim that no two snowflakes are alike is based on the understanding that the basic shape of a snowflake is guided by a combination of atmospheric temperature and humidity. However, its individuality is “crafted” as it falls through the atmosphere, tracing unique paths and being exposed to different air patterns as its shape shifts with each tumble, spin, pitch and glide, and twirl.
Another, perhaps lesser-known example is the shells of diatoms, a type of algae that is characterized as being encased in a shell formed of silica. These silica shells often exhibit a remarkable level of complexity, being extremely porous to permit gas and nutrient exchange with the surrounding environment.
What makes them of particular interest to physicists and materials scientists is their ability to repeatedly produce these intricate and complex shells through self-assembly with such accuracy that the identification and classification of these creatures can be guided by the patterns and placements of their pores.
Until recently, the study of how these patterns emerge has focused on looking at the chemical compositions and material properties of complex microsystems, or has worked on defining the initial conditions required to generate these forms. While the understanding that complex structures are usually created as a result of dynamic interactions with the surrounding environment is well-accepted, how these complex structures form through self-assembly had never yet reached the point of being something scientists could predict.
So Noorduin and co-authors set off undaunted to discover how dynamic environmental interactions can be used to generate predictable, patterned precipitation in synthetic systems, to create self-assembled complex structures… repeatedly (i.e., in a predictable manner).
It turns out that the secret to growing complex structures is to subject the solution to a dynamically-changing environment, one that responds to its growing shape. The images to the left are scanning electron micrographs that have been artificially colored, but aren’t they beautiful?
It’s important to realize that while Noorduin and colleagues did produce these flowers as a beautiful demonstration of their technique, that all of this isn’t purely just for fun and games. Rather, if we can harness the basic principles guiding self-assembly, especially that of biomineralization, this will change the face of how nano- and micromaterials are made.
As of now, our go-to method for creating nanomaterials is by using lithography techniques, through which 3D structures are laboriously etched. Not only is this slow, it is also very, very expensive. Using the methods this group is developing, it will soon become possible to mass-manufacture complex materials for drug delivery, development of catalysts for chemical production, micro-circuitry, etc., by mechanisms of self-assembly.
The possibilities are truly… endless.
- Pictures: Nano “Flowers” Created in Lab (news.nationalgeographic.com)
- Harvard Researches Grow a Garden of Nanoscience Flowers (boston.com)
- Microscopic crystal ‘flowers’ build themselves in a Harvard lab (science.nbcnews.com)
As crazy and sci-fi as it might seem to think of building a computer using cellular components, it was even more shocking to me to learn that the term, “synthetic biology” has a history that goes all the way back to 1910. In fact, in 1974, Polish geneticist Wacław Szybalski used the term to examine the idea of using molecular biology in a synthetic manner to “devise new control elements” in a modular manner for creating new genomes, new organisms, etc… BRILLIANT!!! It took some time for technology — and science — to catch up with such revolutionary thinkers, and synthetic biology finally began to take off in 2000.
Really, the idea of synthetic biology (horribly over-simplified) is based on the recognition that biological organisms — no matter how simple — process their environment remarkably quickly, with a resolution and speed beyond anything we, as engineers, have been capable of achieving so far. Another way to think about this is that in this modern age of BIG DATA, for which our greatest challenges are how to store, manage, and analyze the continuous onslaught of exabytes (hunh??) of data, biological organisms had this figured out… well… millions of years ago. Youtube’s streaming video? Got it! Bose’s Quiet Comfort noise canceling earphones? Remember how we all ignored nagging parents or droning lecturers… oh yes, got that. iPhone’s accelerometers? Yup. And what’s even more inspirational or frustrating (depending on which side of the technological line you fall) is that we don’t only do it faster, we do it better.
Here’s an example. In 2004, in response to a synthetic biology competition call by the International Genetically Engineered Machine (iGEM) Foundation, a group of students at UT Austin invented a method for making photos out of… bacteria! The basic idea is that they genetically-engineered E. coli bacteria to respond to light, giving them a new biological circuit that would cause them to turn black when growing in dark areas and to turn clear in the light. A reasonable analogy would be to think of each bacterium as a pixel on your computer or TV screen. By then spreading and growing them evenly on a Petri dish as a homogeneous lawn of bacteria, and then projecting lighted images on them, the students could reproduce images using these genetically-engineered bacteria.
Why is this exciting? Well, for all of you owners of the latest iPad, iPhones, and MacBook Pros, your stunningly gorgeous Retina displays have approximately 326 PPI (that stands for “pixels per inch”). In contrast, with the bacterial images developed in 2004 (yes, nine years ago!), we’re talking gigapixels per square inch resolution, or thousands of pixels per inch, to make it more comparable to the PPI unit. That’s an order of magnitude greater than the Retina displays… I mean… WOW!
But, ok. So for all of you super-skeptics out there, why should we care? This is a monochromatic display that can (literally) die, when we’re into vibrant, saturated, archival colors seamlessly (sort of) integrated into our electronics… not to mention that these students had to build a seven-foot tall projector to create these relatively simple-looking images… which don’t even move! (Yes, we can all hear the collective gasp by the MTV generation and onwards.) [A side note to all you curmudgeons: I still think this is wonderfully cool.]
Let’s get back to the inspiration for this post. Last week, the journal Nature published an article by Daniel and colleagues from MIT, titled: “Synthetic analog computation in living cells”. While our obsession with the digital processing universe makes this sound like a step back into the Dark Ages, an advantage of analog devices is that they can be simpler while maintaining greater bandwidth and frequency range (yes, think about this from the standpoint of music!). When a signal is converted from analog to digital, it can lose some of its range (or fidelity) depending on the sensors involved, and this conversion process takes time.
Some of you may have heard of the analogy of a biological cell being somewhat like a digital computer, which processes everything as a series of ones and zeros. In reality, however, this is actually a gross oversimplification. While cells do respond to certain stimulants with a binary (on/off) response, the reality is that they use a mix of these digital-like responses and graded responses (think grays, and sorry, I’m not referring to Fifty Shades of Gray) when responding to various inputs — something the digital world of ones and zeros, black and white, cannot do. However, those who have tried to take the cell-computer analogy all the way have been able to use DNA as components for producing digital calculators, sensors and the like. The drawback is that these circuits require an enormous number of components to perform the simplest computations. Each component could be a DNA strand or a protein, which makes the process slower and more difficult to reproduce.
The system that Daniel and colleagues have assembled takes bacterial cells and transforms them into living calculators that can compute logarithms, multiplication, division, and can perform even more sophisticated functions such as acting as an in vivo pH meter… with three or fewer genetic parts. Furthermore, because their system operates in the analog signal processing domain, it can process graded information, characteristic of the natural environment in which we live and with which we interact. Such analog computation could permit the design of cellular sensors for pathogens or toxins. It may now also be possible to combine their analog technology with the digital systems to construct a synthetic digital/analog hybrid system that can swap between the two signal processing approaches according to which mechanism can produce faster or more accurate calculations or processes. Also, from the broader biological context, it now may also be possible to observe the behavior of such a synthetic, analog system and begin to gain a better understanding for how biological systems receive and process complex information, permitting the rapid responses and fine control necessary to make life a brilliant, rapid, high-resolution reality.
OK, so I just stole the title of this post from NSF”s first episode of a series they produced in collaboration with the U.S. Patent and Trademark Office and NBC Learn, to explore innovations… and their innovators, around the US. In a fantastic collection of eleven videos, they cover everything from prosthetic exoskeletons (bionic limbs) and 3-D printing, through smart materials, security, and automation. Ready to learn how these innovators came up with their inventions? Are you ready to be inspired? If so, check out the videos here.